Protein_Domain

Part:BBa_K3619003:Design

Designed by: Isak Ingerholt   Group: iGEM20_Lund   (2020-09-12)


cDNA encoding the protein enterokinase.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 211


Design Notes

The sequence is codon optimized for expression in E. coli K12.


Source

The sequence was retrieved from a paper were enterokinase was successfully expressed and the proteins origin is bovine intestin. [2]

References

1. https://doi.org/10.1016/S1046-5928(02)00012-8

2. https://www.jbc.org/content/268/31/23311.long

3. https://www.uniprot.org/uniprot/E6Y432